产品名称：FITC-labeled Rat Anti-Mouse Integrin a2 Chain (CD49b)

品牌：Emfret

货号：M070-1

品名：Emfret FITC-labeled Rat Anti-Mouse Integrin a2 Chain (CD49b)

规格：1.5 ml / 300 tests

同种型：Sam.G4 / Rat (Wistar) IgG2b

包含：FITC-labeled immunoglobulin in 20 mM Tris buffer with 137 mM NaCl, 0.5% BSA and 0.09% (w/v) sodium azide

特异性：

The Sam.G4 antibody reacts with the mouse integrin a2 chain (CD49b), the 160-kDa transmembrane glycoprotein that non-covalently associates with the integrin b1 subunit to form the integrin a2b1 complex known as VLA-2. Integrin a2b1, a receptor for collagen and laminin, is expressed on platelets, epithelial cells, and activated lymphocytes1,2. The Sam.G4 antibody can block integrin a2b1 binding to collagen3 .

用途：

: The antibody preparation is optimized for flow cytometric applications: Use 5 µl to stain ~106 platelets or ~0.5x106 cells in a recommended volume of 25 µl. Incubate for 15 minutes at room temperature, stop reaction by addition of 400 µl PBS and analyze samples within 30 minutes. For immunofluorescent staining of acetone-fixed frozen sections, the appropriate dilution must be determined individually. 

制备和储存：

The antibody was purified from hybridoma cell culture supernatant by Protein G-Sepharose chromatography. The antibody was conjugated with FITC under optimum conditions. The solution is free of unbound FITC. Store product undiluted at 4°C and avoid prolonged exposure to light. Stable for one year from date of shipment. Do not freeze.

注意：

Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer.

注：仅供研究使用，不用于诊断或治疗用途。