产品名称：Emfret Purified Rat Anti-Mouse GPVI

品牌：Emfret

货号：M011-0

品名：Emfret Purified Rat Anti-Mouse GPVI

规格：0.5 mg/ml 

克隆：JAQ1/ Rat (Wistar) IgG2a

包含：0.1 mg purified immunoglobulin in 20 mM Tris buffered saline containing 0.25% BSA and 0.09% (w/v) sodium azide

特异性：

 The JAQ1 antibody reacts with mouse GPVI, a platelet/megakaryocyte-specific 60 to 65 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily1 . GPVI noncovalently associates with the signal-transducing FcR-chain in the platelet membrane and serves as an activating collagen receptor2 . JAQ1 inhibits collagen-induced aggregation of mouse platelets3 . JAQ1 alone does not activate platelets. Cross-linking of JAQ1 by a secondary antibody, however, induces activation and aggregation of mouse platelets3 . 

用途：

This preparation can be used for immunoprecipitation, immunohistochemical analysis of acetone-fixed frozen sections, and immunofluorescent staining (1-3 µg/106 platelets or cells). JAQ1 recognizes mouse GPVI in a Western blot analysis under non-reducing condtions3 . For varying applications, appropriate dilutions must be determined individually.

制备和储存：

The antibody was purified from hybridoma cell culture supernatant by Protein G-Sepharose chromatography. Stable for six months from date of shipment when stored at 4°C. Aliquots can be stored at -20°C for at least one year. Avoid repeated freezing and thawing.

注意：

Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer

注：仅供研究使用，不用于诊断或治疗用途。