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  • 产品名称:Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody, Histone Gamma-H2A.X (Phospho S139) Clone RM224

  • 产品型号:31-1104-00
  • 产品厂商:RevMAb
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Product Details
Product Name Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody [RM224]
Product Description Rabbit monoclonal to Gamma H2AX (phospho-Ser139)
Catalog No. 31-1104-00
Clone Name RM224
Product Data Sheet Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody Data Sheet RM224
Specificity RM224 reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones.
Immunogen A phospho-peptide corresponding to Phospho-Histone H2AX (Ser139).
Application Western Blot (WB)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt.
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 ug/mL – 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 1 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL.

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Product Images

Western Blot of acid extracts of HeLa cells treated
or non-treated with UV. Using RM224 at 0.5 ug/mL,
showed a band of Histone H2A.X phosphorylated
at serine 139 in HeLa cells.

Immunocytochemistry of HeLa cells using gamma
H2AX Rabbit mAb RM224 (red). Actin filaments
have been labeled with fluorescein phalloidin (green).


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