Anti-Acetyl-Histone H3 (Lys18) Rabbit Monoclonal Antibody, Clone RM166; H3K18ac, Histone H3 (K18 acetylation) 31-1055-00 进口试剂采购网

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产品名称：Anti-Acetyl-Histone H3 (Lys18) Rabbit Monoclonal Antibody, Clone RM166; H3K18ac, Histone H3 (K18 acetylation)
产品型号：31-1055-00
产品厂商：RevMAb
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Product Details
Product Name	Anti-Acetyl-Histone H3 (Lys18) Rabbit Monoclonal Antibody [RM166]
Product Description	Rabbit monoclonal to Acetylated Histone H3 Lysine 18 (K18ac), H3K18ac, Histone H3 (K18 acetylation)
Catalog No.	31-1055-00
Clone Name	RM166
Product Data Sheet	Anti-Acetyl-Histone H3 Lys18 Rabbit Monoclonal Antibody Data Sheet RM166
Specificity	RM166 reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with other acetylated Lysines in histone H3.
Immunogen	An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys18).
Application	Western Blot (WB) Chromatin IP (ChIP) Immunocytochemistry (ICC) Immunohistochemistry (IHC) ELISA Multiplex
Species Reactivity	Human Vertebrates
Conjugate	None
Intended Use	Research Use Only
.
Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	100 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Western Blot: 0.5 ug/mL - 2 ug/mL; ICC: 0.5 ug/mL - 2 ug/mL; ChIP: 2 ug/mL-10 ug/mL; IHC: 1 ug/mL-10 ug/mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.1 ug/mL – 0.5 ug/mL.
Keywords
References for Acetyl-Histone H3 (Lys18) antibody [RM166]	Li Y. et al. Activation of mutant TERT promoter by RAS-ERK signaling is a key step in malignant progression of BRAF-mutant human melanomas. Proc Natl Acad Sci USA. 113(50):14402-14407. ChIP; Human. Read more (PubMed: 27911794)
Product Images
	RM166 specifically reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79 (K79ac), or Lysine 122 (K122) in Histone H3.
	Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using RM166 at 0.5 ug/mL, showed a band of histone H3 acetylated at Lysine 18 in treated HeLa.
	Sandwich ELISA against acetylated histone H3 at Lys 18 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using anti-H3CT (RM188, 1 ug/mL) as the capture antibody and biotinylated anti-H3K18C (RM166, 2 ug/mL) as the detection antibody.
	ChIP performed on HeLa cells with or without Sodium Butyrate treatment, using H3K18Ac antibody (RM166, 5ug). Real-time PCR was performed using primers specific to the geneindicated.
	Immunocytochemistry of HeLa cells treated withsodium butyrate, using Acetyl-Histone H3 (Lys18) Rabbit mAb RM166(red). Actin filaments havebeen labeled with fluorescein phalloidin (green).
	Immunohistochemistry staining of HepG2 cells using Anti-Acetyl-Histone H3 (Lys18) antibody, RM166.