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  • 产品名称：Biotin Anti-Human IgG Rabbit Monoclonal Antibody, Clone RM116

  • 产品型号：31-1018-02
  • 产品厂商：RevMAb
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Product Details
Product Name Biotin Anti-Human Gamma Heavy Chain Rabbit Monoclonal Antibody [RM116]
Product Description Biotinylated Rabbit Monoclonal to Human Gamma Heavy Chain
Catalog No. 31-1018-02
Clone Name RM116
Product Data Sheet Biotin Anti-Human Gamma Heavy Chain Rabbit Monoclonal Antibody Data Sheet RM116
Specificity RM116 reacts to the Fc region of all gamma heavy chains of human immunoglobulins, including γ1, γ2, γ3, and γ4. No cross reactivity with human IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Immunogen Human IgG
Application Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species Reactivity Human
Conjugate Biotin
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 50 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 50ng/well – 200ng/well (for Capture);
0.05ug/mL – 0.2ug/mL (for Detection);
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
Keywords
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Product Images
Immunohistochemistry

Immunohistochemistry of Human Tonsil using
 Anti-Gamma Heavy Chain antibody RM116.

ELISA

ELISA of human immunoglobulins shows RM116
reacted to the γ1, γ2, γ3, γ4 heavy chain of human
IgGs, and the Fc of human IgG. No cross reactivity with
other human heavy chains, mouse IgG, rat IgG,
or goat IgG. The plate was coated with 50 ng/well
of different immunoglobulins. 200 ng/mL or 50 ng/mL
of RM116 was used as the primary antibody. An
alkaline phosphatase conjugated anti-rabbit IgG as the
secondary antibody.

A

A titer ELISA using RM116. The plate was coated
 with different amounts of human IgG1. A serial dilution
 of RM116 was used as the primary antibody. An alkaline
 phosphatase conjugated anti-rabbit IgG as the secondary
 antibody.

Sandwich ELISA using RM116 as the capture antibody
 (100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody (1ug/mL),
 followed by an alkaline phosphatase conjugated streptavidin.

Sandwich ELISA using RM116 as the capture antibody
 (100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody (1ug/mL),
 followed by an alkaline phosphatase conjugated streptavidin.


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