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  • 入驻时间: 2012-04-17
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产品详情
  • 产品名称:Anti-Mouse IgG1 Rabbit Monoclonal Antibody, Clone RM106

  • 产品型号:31-1002-00
  • 产品厂商:RevMAb
  • 产品价格:0
  • 折扣价格:0
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Product Details
Product Name Anti-Mouse IgG1 Rabbit Monoclonal Antibody [RM106]
Product Description Rabbit monoclonal to Mouse IgG1
Catalog No. 31-1002-00
Clone Name RM106
Product Data Sheet Anti-Mouse IgG1 Rabbit Monoclonal Antibody Data Sheet RM106
Specificity RM106 reacts to the Fc region of mouse IgG1. No cross reactivity with mouse IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.

The Fc region of RM106 has been engineered to eliminate Fc receptor binding.
Immunogen Mouse IgG
Application Western Blot (WB)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species Reactivity Mouse
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 0.005 ug/mL-0.2 ug/mL;
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL;
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL;
Western Blot (WB): 0.1 ug/mL-0.5 ug/mL.
Keywords
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Product Images
Western

Western blot of nonreduced(-) and reduced(+)
mouse IgG1 (20ng/lane), using 0.2ug/mL of
RevMAb clone RM106. This antibody reacts to
nonreduced IgG1 (~150 kDa) stronger than the
reduced γ1 form (~50 kDa).

ELISA

ELISA of mouse immunoglobulins shows RM106
reacts to the Fc region of mouse IgG1; no cross
reactivity with IgG2a, IgG3, IgM, IgA, IgE, human
IgG, or rat IgG. The plate was coated with 50 ng/well
of different immunoglobulins. 200 ng/mL, 50 ng/mL,
or 10 ng/mL of RM106 was used as the primary
antibody. An alkaline phosphatase conjugated anti-
rabbit IgG as the secondary antibody.

A

A titer ELISA of mouse IgG1. The plate was coated
with different amount of mouse IgG1. A serial dilution
of RM106 was used as the primary antibody. An
alkaline phosphatase conjugated anti-rabbit IgG as
the secondary antibody.


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