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| Product Details | |
| Product Name | Anti-Dimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody [RM181] |
| Product Description | Rabbit monoclonal to Dimethylated Histone H3 Lysine 79, H3K79me2, Histone H3 (dimethyl K79) |
| Catalog No. | 31-1065-00 |
| Clone Name | RM181 |
| Product Data Sheet | Anti-Dimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody Data Sheet RM181 |
| Specificity | RM181 reacts to Histone H3 dimethylated at Lysine 79 (K79me2). ). Very slightly cross-reacts with monomethylated Lysine 79 (K79me1) at high concentration. No cross reactivity with non-modified Lysine 79 (K79 ctrl), trimethylated Lysine 79 (K79me3), or other methylations in Histone H3. |
| Immunogen | A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys79). |
| Application |
Western Blot (WB) Chromatin IP (ChIP) Immunohistochemistry (IHC) ELISA Multiplex |
| Species Reactivity |
Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
| Properties | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 ug |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage |
Western Blot: 0.25 ug/mL – 1 ug/mL; IHC: 0.1 ug/mL – 1 ug/mL; ChIP: 2 ug/mL- 10 ug.mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.1 ug/mL – 0.5 ug/mL. |
| Keywords | |
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| Product Images | |
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RM181 specifically reacts to Histone H3 dimethylated |
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Western Blot of recombinant histone H3.3 (1) |
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ChIP performed on HeLa cells using H3K79me2 antibody |
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Immunohistochemistry staining of HepG2 cells using |
