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第12年
- 入驻时间： 2012-04-17
- 联系人：客户经理
- 电话：400-968-7988
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联系时，请说明易展网看到的
- Email：fuwu@bioleaf.com
产品详情
简单介绍：
详情介绍：
| Product Details | |
| Product Name | Anti-EGFR Variant III Rabbit Monoclonal Antibody [RM419] |
| Product Description | Rabbit monoclonal to Epidermal Growth Factor Receptor Variant III |
| Catalog No. |
31-1305-00 31-1305-02 |
| Clone Name | RM419 |
| Product Data Sheet |
Anti-EGFR Variant III Rabbit Monoclonal Antibody Data Sheet RM419 Biotinylated Anti-EGFR Variant III Rabbit Monoclonal Antibody Data Sheet RM419 |
| Specificity | RM419 reacts to human Epidermal Growth Factor Receptor Variant III. No cross reactivity with wild type EGF receptor. |
| Immunogen | A peptide corresponding to epidermal growth factor receptor variant III (EGFRvIII) |
| Application |
IHC, WB, ELISA, FC |
| Species Reactivity |
Human |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
| Properties | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size |
100 uL; Biotinylated 50 uL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage |
IHC: 1:100 – 1:200 dilution; FC: 1:10 – 1:100 dilution; WB: 1:1000 - 1:2000 dilution; ELISA: 1:100 – 1:1000 dilution. |
| Keywords | |
| . | |
| Product Images | |
|
Immunohistochemical staining of formalin fixed and paraffin embedded human Glioblastoma Multiforme tissue sections with or without EGFRvIIII using anti-EGFRvIII antibody (RM419) at 1:100 dilution. |
|
Western Blot analysis of DKMG (EGFRvIII positive), U118MG, A431, and HeLa whole cell lysates, using anti-EGFRvIII antibody (RM419) and anti-EGFRct (RM294) at 1:1000 dilution. |
|
Flow cytometry analysis of DKMG and 293 cells, using anti-EGFRvIII antibody (RM419) at 1:50 dilution. |
|
ELISA analysis showing RM419 does not react to wild type EGFR protein. The plate was coated with recombinant EGFR variant III and EGFRwt proteins. A serial dilution of anti-EGFRvlll (RM419) or anti-EGFR (RM229) antibodies were used. |
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