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  • 入驻时间: 2012-04-17
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  • 产品名称:Anti-Acetyl-Histone H3 (Lys23) Rabbit Monoclonal Antibody, Clone RM169; H3K23ac, Histone H3 (K23 acetylation)

  • 产品型号:31-1087-00
  • 产品厂商:RevMAb
  • 产品价格:0
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Product Details
Product Name Anti-Acetyl-Histone H3 (Lys523) Rabbit Monoclonal Antibody [RM169]
Product Description Rabbit monoclonal to Acetylated Histone H3 Lysine 23 (K23ac), H3K23ac, Histone H3 (K23 acetylation)
Catalog No. 31-1087-00
Clone Name RM169
Product Data Sheet Anti-Acetyl-Histone H3 Lys23 Rabbit Monoclonal Antibody Data Sheet RM169
Specificity RM169 reacts to Histone H3 acetylated at Lysine 23 (K23ac). No cross reactivity with other acetylated Lysines in histone H3.
Immunogen An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys23).
Application Western Blot (WB)
ELISA
Multiplex
Immunocytochemistry (ICC)
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.5 ug/mL - 1 ug/mL;
Multiplex: 0.2 ug/mL – 1 ug/mL;
Immunocytochemistry: 1 ug/mL- 2 ug/mL.

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Product Images
Western

Western Blot of acid extracts of HeLa cells non-treated
(1) or treated with sodium butyrate (2), and recombinant
histone H3.3 (3), using RM169 at 1 ug/mL, showed a
band of histone H3 acetylated at Lysine 23 in treated HeLa.

RM169

RM169 specifically reacts to Histone H3 acetylated at Lysine 23
(K23ac). No cross reactivity with unmodified Lysine 23 (K23 ctrl),
acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac),
Lysine 18 (K18ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56
(K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.

Immunocytochemical

Immunocytochemical staining of HeLa cells treated
with sodium butyrate, using anti-Acetyl-Histone
H2A.Z (Lys4) Rabbit Monoclonal Antibody (clone
RM221) (red). Actin filaments have been labeled
with fluorescein phalloidin (green).


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