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| Product Details | |
| Product Name | Anti-Acetyl-Histone H3 (Lys56) Rabbit Monoclonal Antibody [RM179] |
| Product Description | Rabbit monoclonal to Acetylated Histone H3 Lysine 56 (K56ac), H3K56ac, Histone H3 (K56 acetylation) |
| Catalog No. | 31-1078-00 |
| Clone Name | RM179 |
| Product Data Sheet | Anti-Acetyl-Histone H3 Lys56 Rabbit Monoclonal Antibody Data Sheet RM179 |
| Specificity | RM179 reacts to Histone H3 acetylated at Lysine 56 (K56ac). No cross reactivity with other acetylated Lysines in histone H3. |
| Immunogen | An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys56). |
| Application |
Western Blot (WB) Immunocytochemistry (ICC) Immunohistochemistry (IHC) Chromatin IP (ChIP) ELISA Multiplex |
| Species Reactivity |
Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
| Properties | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 ug |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage |
Western Blot: 1 ug/mL - 2 ug/mL; ICC: 0.5 ug/mL - 2 ug/mL; IHC: 1 ug/mL – 10 ug/mL; ELISA: 0.5 ug/mL - 1 ug/mL; Multiplex: 0.5 ug/mL – 2 ug/mL. |
| Keywords | |
| References for Acetyl-Histone H3 (Lys56) antibody [RM179] | Li Y. et al. Activation of mutant TERT promoter by RAS-ERK signaling is a key step in malignant progression of BRAF-mutant human melanomas. Proc Natl Acad Sci USA. 113(50):14402-14407. ChIP; Human. Read more (PubMed: 27911794) |
| Product Images | |
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Western Blot of (1) acid extracts of HeLa cells treated with |
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RM179 specifically reacts to Histone H3 acetylated at Lysine 56 |
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Immunocytochemistry of HeLa cells treated with |
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Immunohistochemistry staining of HepG2 cells using |
