Anti-Human IgG1 Rabbit Monoclonal Antibody, Clone RM117 31-1019-00 进口试剂采购网

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产品名称：Anti-Human IgG1 Rabbit Monoclonal Antibody, Clone RM117
产品型号：31-1019-00
产品厂商：RevMAb
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Product Details
Product Name	Anti-Human IgG1 Rabbit Monoclonal Antibody [RM117]
Product Description	Rabbit monoclonal to Human IgG1
Catalog No.	31-1019-00
Clone Name	RM117
Product Data Sheet	Anti-Human IgG1 Rabbit Monoclonal Antibody Data Sheet RM117
Specificity	RM117 reacts to the heavy chain of human IgG1. RM117 does not cross react to any other IgG subclasses (IgG2, IgG3, or IgG4), and shows no cross reactivity to IgM, IgA, IgD, IgE. RM117 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Immunogen	Peptide corresponding to the hinge region of Human IgG1
Application	Immunohistochemistry (IHC) Flow Cytometry (FC) Immunocytochemistry (ICC) ELISA
Species Reactivity	Human
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size	100ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	ELISA: 50ng/well – 200ng/well (for Capture); 0.05ug/mL – 0.2ug/mL (for Detection); Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL; Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
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Product Images
	Detection of human IgG1 in monkey serum, using RM117 (capture) and biotin-RM129 (detection) as a Sandwich ELISA pair. HRP conjugated streptavidin and TMB were used to yield the colorimetric reaction.
	Sandwich ELISA using RM117 as the capture antibody, and biotinylated anti-human light chains( κ+ λ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin.
	ELISA showing RM117 reacts only to human IgG1, and not to any other IgG subclasses (IgG2, IgG3, or IgG4), and no cross reactivity to IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
	A titer ELISA using RM117. The plate was coated with different amounts of human IgG1. A serial dilution of RM117 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
	ELISA showing RM117 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM117 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
	Immunohistochemical staining of FFPE Tonsil tissue sections using Anti-Human IgG1 antibody RM117 at a 1:15,000 dilution. Image courtesy of Shaomin Hu, MD, PhD, Department of Anatomic and Clinical Pathology. Montefiore Medical Center Albert Einstein College of Medicine. Bronx, New York, USA