Anti-Human IgG4 Rabbit Monoclonal Antibody, Clone RM120 31-1022-00 进口试剂采购网

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产品名称：Anti-Human IgG4 Rabbit Monoclonal Antibody, Clone RM120
产品型号：31-1022-00
产品厂商：RevMAb
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Product Details
Product Name	Anti-Human IgG4 Rabbit Monoclonal Antibody [RM120]
Product Description	Rabbit monoclonal to Human IgG4
Catalog No.	31-1022-00
Clone Name	RM120
Product Data Sheet	Anti-Human IgG4 Rabbit Monoclonal Antibody Data Sheet RM120
Specificity	RM120 reacts to the heavy chain of human IgG4, RM120 does not cross react to any other IgG subclasses (IgG1, IgG2, or IgG3), and shows no cross reactivity to IgM, IgA, IgD, IgE. RM120 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Immunogen	Peptide corresponding to the hinge region of Human IgG4
Application	Immunohistochemistry (IHC) Flow Cytometry (FC) Immunocytochemistry (ICC) ELISA Western Blot
Species Reactivity	Human
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size	100ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	ELISA: 50ng/well – 200ng/well (for Capture); 0.05ug/mL – 0.2ug/mL (for Detection); Western Blot (WB): 0.5 ug/mL – 2 ug/mL; Immunocytochemistry (ICC): 1 ug/mL-10ug/mL; Immunohistochemistry (IHC): 1 ug/mL-10ug/mL.
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Product Images
	Detection of human IgG4 in monkey serum.Sandwich ELISA using RM120 as the capture antibody, and biotinylated anti-human light chains ( κ+ λ) antibody RM129 as the detection antibody, followed by a HRP conjugated streptavidin.
	Sandwich ELISA using RM120 as the capture antibody, and biotinylated anti-human light chains( κ+ λ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin.
	Western Blot of human, mouse, rat, and goat IgG shows RM120 reacts to human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms. No cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG.
	A titer ELISA using RM120. The plate was coated with different amounts of human IgG4. A serial dilution of RM120 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgGas the secondary antibody.
	Immunohistochemical staining of formalin fixed and paraffin embedded human lymphoid tissue section using anti-human IgG4 rabbit monoclonal antibody (Clone RM120).
	ELISA showing RM120 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM120 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
	Immunohistochemical staining of FFPE Tonsil tissue sections using Anti-Human IgG4 antibody RM120 at a 1:50,000 dilution. Image courtesy of Shaomin Hu, MD, PhD, Department of Anatomic and Clinical Pathology. Montefiore Medical Center Albert Einstein College of Medicine. Bronx, New York, USA