Anti-Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody, Clone RM130; H3K14ac, Histone H3 (K14 acetylation) 31-1032-00 进口试剂采购网

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产品名称：Anti-Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody, Clone RM130; H3K14ac, Histone H3 (K14 acetylation)
产品型号：31-1032-00
产品厂商：RevMAb
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Product Details
Product Name	Anti-Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody [RM130]
Product Description	Rabbit monoclonal to Acetylated Histone H3 Lysine 14 (K14ac), H3K14ac, Histone H3 (K14 acetylation)
Catalog No.	31-1032-00
Clone Name	RM130
Product Data Sheet	Anti-Acetyl-Histone H3 Lys14 Rabbit Monoclonal Antibody Data Sheet RM130
Specificity	RM130 reacts to Histone H3 acetylated at Lysine 14 (K14ac), and is not affected by the modification of neighboring amino acids. No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), or lysine 79 (K79ac) in histone H3.
Immunogen	An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys14)
Application	Western Blot (WB) Immunocytochemistry (ICC) Chromatin IP (ChIP) ELISA Multiplex
Species Reactivity	Human Vertebrates
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	100 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Western Blot: 0.5 ug/mL - 2 ug/mL; ICC: 0.5 ug/mL - 2 ug/mL; ChIP: 2 ug/mL- 10 ug/mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.1 ug/mL – 0.5 ug/mL.
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Product Images
	RM130 specifically reacts to Histone H3 acetylated at Lysine 14 (K14ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), or lysine 79 (K79ac) in histone H3.
	The binding specificity of RM130 to Histone H3 acetylated at Lysine 14 (K14ac) is not affected by the modification of neighboring amino acids.
	Western Blot of acid extracts from HeLa cellsuntreated (-) or treated with sodium butyrate (+) or, using RM130 at 0.5 ug/mL.
	ChIP performed on HeLa cells using H3K14ac antibody (RM130, 5ug). Real-time PCR was performed using primers specific to the gene indicated.
	Immunocytochemistry of HeLa cells treated withsodium butyrate, using Acetyl-Histone H3 (Lys14) Rabbit mAb RM130 (red). Actin filaments havebeen labeled with fluorescein phalloidin (green).