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Product Details | |
Product Name | Anti-Histone H3 K27M Rabbit Monoclonal Antibody [RM192] |
Product Description | Rabbit monoclonal to Histone H3 K27M; Histone H3, K27M Mutant |
Catalog No. | 31-1175-00 |
Clone Name | RM192 |
Product Data Sheet | Anti-Histone H3 K27M Rabbit Monoclonal Antibody Data Sheet RM192 |
Specificity | RM192 reacts to the Histone H3 K27M mutant. No cross reactivity with wild type Histone H3. |
Immunogen | A peptide corresponding to Histone H3 K27M mutant |
Application |
Western Blot ELISA Immunohistochemistry Immunocytochemistry |
Species Reactivity | All |
Conjugate | None |
Intended Use | Research Use Only |
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Properties | |
Form | Liquid |
Storage Instructions | Store at -20.0°C |
Stability | Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Volume/Size | 50 ug |
Concentration | 1.0 mg/mL |
Purity | Protein A affinity purified from an animal origin–free culture supernatant |
Clone Type | Monoclonal |
Isotype | Rabbit IgG |
Usage |
Western Blot: 0.01 ug/mL – 1 ug/mL; ELISA: 0.5 ug/mL - 2 ug/mL; Immunohistochemistry (IHC): 0.005 – 0.1 ug/mL; Immunocytochemistry (ICC/IF): 0.005 – 0.1 ug/mL. |
References for Histone H3 K27M Mutant antibody [RM192] |
Pathania et al. H3.3K27M Cooperates with Trp53 Loss and PDGFRA Gain in Mouse Embryonic Neural Progenitor Cells to Induce Invasive High-Grade Gliomas Cancer Cell (2017)10.1016/j.ccell.2017.09.014. IHC-P; Human. Read more (PubMed: 29107533) |
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Product Images | |
Immunostaining of brain tumor tissue sections
Image courtesy of Sebastian Brandner MD, Division |
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Immunostaining of brain tumor tissue sections
Image courtesy of Sebastian Brandner MD, Division |
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Western Blot analysis of cell lysates prepared from |
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Immunohistochemical staining of formalin fixed |