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| Product Details | |
| Product Name | Anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody [RM186] |
| Product Description | Rabbit Monoclonal to Histone H3 (Unmodified Lys4); Histone H3 (Unmodified Lysine 4) |
| Catalog No. | 31-1086-00 |
| Clone Name | RM186 |
| Product Data Sheet | Anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody Data Sheet RM186 |
| Specificity | RM186 recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated or methylated Lys4. The antibody binding specificity allows for modifications of Arg2, Thr3, and/or other modifications in Histone H3. |
| Immunogen | An unmodified peptide corresponding to the N-terminus of Histone H3. |
| Application |
Western Blot (WB) ELISA Multiplex Immunocytochemistry (ICC) |
| Species Reactivity |
Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
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| Properties | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 ug |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage |
Western Blot: 1 ug/mL – 2 ug/mL; ELISA: 0.5 ug/mL - 1 ug/mL; Multiplex: 0.5 ug/mL – 2 ug/mL; Immunocytochemistry: 0.5 ug/mL- 2 ug/mL. |
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| Product Images | |
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RM186 specifically recognizes Histone H3 unmodified |
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Western Blot of acid extracts of HeLa cells treated with |
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Immunocytochemical staining of HeLa cells treated |
