Biotin Anti-Human IgG3 Rabbit Monoclonal Antibody, Clone RM119 31-1021-02 进口试剂采购网

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产品名称：Biotin Anti-Human IgG3 Rabbit Monoclonal Antibody, Clone RM119
产品型号：31-1021-02
产品厂商：RevMAb
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Product Details
Product Name	Biotin Anti-Human IgG3 Rabbit Monoclonal Antibody [RM119]
Product Description	Biotinylated Rabbit monoclonal to Human IgG3
Catalog No.	31-1021-02
Clone Name	RM119
Product Data Sheet	Biotin Anti-Human IgG3 Rabbit Monoclonal Antibody Data Sheet RM119
Specificity	RM119 reacts to the heavy chain of human IgG3. No cross reactivity with human IgG1, IgG2, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Immunogen	Human IgG3
Application	Immunohistochemistry (IHC) Flow Cytometry (FC) Immunocytochemistry (ICC) ELISA
Species Reactivity	Human
Conjugate	Biotin
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size	50 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	ELISA: 50ng/well – 200ng/well (for Capture); 0.05ug/mL – 0.2ug/mL (for Detection); Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL; Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
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Product Images
	Immunohistochemistry of Human Lymphoid Tissue using Anti-Human IgG3 antibody RM119.
	Sandwich ELISA using RM119 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody RM129 as the detection antibody, followed by an HRP conjugated streptavidin.
	Sandwich ELISA using RM119 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody RM129 as the detection antibody, followed by an HRP conjugated streptavidin.
	ELISA of human immunoglobulins shows RM119 reacts only to human IgG3. No cross reactivity with Human IgG1, IgG2, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM119 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
	A titer ELISA using RM119. The plate was coated with different amounts of human IgG3. A serial dilution of RM124 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.