Anti-5-methylcytosine (5-mC) Rabbit Monoclonal Antibody, Clone RM231 31-1110-00 进口试剂采购网

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产品名称：Anti-5-methylcytosine (5-mC) Rabbit Monoclonal Antibody, Clone RM231
产品型号：31-1110-00
产品厂商：RevMAb
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Product Details
Product Name	Anti-5-methylcytosine (5-mC) Rabbit Monoclonal Antibody [RM231]
Product Description	Rabbit monoclonal to 5-methylcytosine (5-mC) Rabbit Monoclonal Antibody, 5'-mC
Catalog No.	31-1110-00
Clone Name	RM231
Product Data Sheet	Anti-5-methylcytosine (5-mC) Rabbit Monoclonal Antibody Data Sheet RM231
Specificity	RM231 reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA.
Immunogen	BSA-conjugated 5-methylcytosine.
Application	Methylated DNA immunoprecipitation (MeDIP) Immunohistochemistry (IHC) Immunocytochemistry (ICC) ELISA Dot Blot (DB)
Species Reactivity	All
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt.
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	50 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Dot Blot: 0.5 ug/mL – 2 ug/mL; ELISA: 0.1 ug/mL - 1 ug/mL; ICC: 0.5 ug/mL - 2 ug/mL; MeDIP: 0.2 ug/mL- 2 ug/mL.
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Product Images
	ELISA of single stranded DNA using rabbit monoclonal anti-5-mC (clone RM231) antibody. The plate was coatedwith streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution of RM231 was used as the primary antibody, and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
	Dot blot of double stranded DNA using rabbit monoclonal anti-5-mC (clone RM231) antibody. The membrane waspre-spotted with 50, 5, and 0.5 ng/dot of double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA. The pre-spotted membrane was then blotted with RM231.
	Immunocytochemical staining of HeLa cells using rabbit monoclonal anti-5-mC (clone RM231) antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).
	Direct ELISA of HeLa cell genomic DNA using anti-5-mC antibody (RM231). The plate was directly coated with different concentrations of genomic DNA isolated from HeLa cells. 1 ug/mL or 3 ug/mL of RM231 was used as the primary antibody, and a HRP conjugated anti-rabbit IgG as the secondary antibody.
	MeDIP was performed using anti-5-mC antibody (RM231) at a 2:1 DNA:Ab ratio. 1 ng of unmethylated, 5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine (5-hmC) DNA standard (897 bp) was spiked in 1ug of genomic DNA isolated from HeLa cells as the control. Realtime PCR was then performed to determine the capture of DNA standard as in % of input.