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  • 入驻时间: 2012-04-17
  • 联系人:客户经理
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产品详情
  • 产品名称:Biotin Anti-Mouse IgG Rabbit Monoclonal Antibody, Clone RM104

  • 产品型号:31-1001-02
  • 产品厂商:RevMAb
  • 产品价格:0
  • 折扣价格:0
  • 产品文档:
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Product Details
Product Name Biotin Anti-Mouse IgG Rabbit Monoclonal Antibody [RM104]
Product Description Biotinylated Rabbit monoclonal to Mouse IgG
Catalog No. 31-1001-02
Clone Name RM104
Product Data Sheet Biotin Anti-Mouse IgG Rabbit Monoclonal Antibody Data Sheet RM104
Specificity RM104 reacts to the Fc region of all mouse IgG subclasses. No cross reactivity with mouse IgM, IgA, IgE, human IgG, and rat IgG. It may cross react to goat IgG.
The Fc region of RM104 has been engineered to eliminate Fc receptor binding.
Immunogen Mouse IgG
Application Western Blot (WB) (nonreduced)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species Reactivity Mouse
Conjugate Biotin
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 50ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 0.005 ug/mL-0.2 ug/mL;
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL;
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL;
Western Blot (WB): 0.1 ug/mL-0.5 ug/mL.
Keywords
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Product Images
Western

Western blot of nonreduced(-) and reduced(+)
mouse immunoglobulins (20ng/lane), using
0.2ug/mL of RevMAb clone RM104. This antibody
reacts to nonreduced mouse IgG1, IgG2a, IgG2b,
and IgG3. It showed no cross reactivity with IgM,
IgA, or IgE.

ELISA

ELISA of mouse immunoglobulins shows RM104
reacts to the Fc region of mouse IgG1, IgG2a,
IgG2b, and IgG3; no cross reactivity with IgM,
IgA, IgE, human or rat IgG. The plate was coated
with 50 ng/well of different immunoglobulins.
200 ng/mL, 50 ng/mL, or 10 ng/mL of RM104 was
used as the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary antibody.

A

A titer ELISA of mouse IgG. The plate was coated
with different amounts of mouse IgG. A serial dilution
of RM104 was used as the primary antibody. An
alkaline phosphatase conjugated anti-rabbit IgG as
the secondary antibody.


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