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  • 产品名称:RAP1A Human

  • 产品型号:
  • 产品厂商:Prospec
  • 产品价格:50
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简单介绍:
RAP1A Human
详情介绍:

Catalogue number

PRO-848

Synonyms

KREV1, G-22K, C21KG, RAP1A, RAP1, Ras-related protein Rap-1A, GTP-binding protein smg p21A, Ras-related protein Krev-1.

Introduction

RAP1A is part of the family of RAS-related proteins which possess 50% amino acid identity with the classical RAS proteins and have various structural characteristics in common. RAP proteins differ from RAS proteins in their 61st amino acid, glutamine in RAS is replaced by threonine in RAP proteins. RAP1A induces morphological reversion of a cell line transformed by a Ras oncogene and counteracts the mitogenic function of Ras, since it can interact with Ras GAPs and RAF in a competitive manner.

Description

RAP1A Recombinant E.coli produced in E.Coli is a single, non-glycosylated polypeptide chain containing 201 amino acids (1-181 a.a.) and having a molecular mass of 22.8 kDa. The RAP1A is fused to 20 amino acid His-Tag at N-terminus and purified by conventional chromatography.

Source

Escherichia Coli.

Physical Appearance

Sterile filtered colorless solution.

Formulation

0.25mg/ml solution containing 20mM Tris HCL pH-8.0, 5mM DTT, 0.2M NaCl & 40% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Purity

Greater than 85.0% as determined by SDS-PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDCQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTEDVP MILVGNKCDL EDERVVGKEQ GQNLARQWCN CAFLESSAKS KINVNEIFYD LVRQINRKTP VEKKKPKKKS C.

Safety Data Sheet

SDS

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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