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  • 入驻时间: 2012-04-17
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  • 产品名称:HIV-1 Integrase p31

  • 产品型号:
  • 产品厂商:Prospec
  • 产品价格:150
  • 折扣价格:0
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简单介绍:
HIV-1 Integrase p31
详情介绍:

Catalogue number

HIV-145

Introduction

Integrase is an enzyme produced by the HIV which enables its genetic material to be integrated into the DNA of the infected cell and is a key component in the pre-integration complex. HIV integrase contains 3 domains, an N-terminal HH-CC zinc fingerdomainwhich is partially responsible for multimerization, a central catalytic domain and a C-terminal domain. Both Central catalytic domain and C-terminal domains have been shown to bind both viral and cellular DNA. No crystal structure data exists with Integrase bound to its DNA substrates. HIV-1 integrase functions as a dimeror a tetramer. Additionally, several host cellular proteins interact with integrase and may facilitate the integration process.

Description

The E.coli derived recombinant protein is a non-glycosylated polypeptide chain, containing the HIV-1 immunodominant regions from the p31 protein (integrase) 9-289 amino acids, fused with  six histidines at N-terminus.

Source

Escherichia Coli.

Physical Appearance

Sterile filtered colorless clear solution.

Formulation

1.5M urea, 25mM Tris-HCl pH 8.0, 0.2% Triton-X & 50% Glycerol.

Purity

Greater than 95.0% as determined by HPLC analysis and SDS-PAGE.

Stability

HIV-1 Integrase p31 although stable at 4°C for 1 week, should be stored below -18°C.
Please prevent freeze thaw cycles.

Specificity

Immunoreactive with all sera of HIV-1 infected individuals.

Applications

HIV-1 Integrase p31 antigen is suitable for ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.

Safety Data Sheet

SDS

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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