Catalogue number
PRO-412
Synonyms
Apoptosis regulator BAX membrane isoform alpha, Bax, Bcl2-associated X protein.
Introduction
Bcl-2–associated X protein (Bax) belongs to the Bcl-2 protein family. Bax is a pro-apoptotic Bcl-2 protein containing BH1, BH2 and BH3 domains. Bax accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor bcl2 or its adenovirus homolog e1b 19k protein. Bax induces the release of cytochrome c, activation of casp3, and thereby apoptosis. Bax is expressed in a wide variety of tissues, with highest levels in the testis and ovary. Tumor suppressor protein p53 upregulates the expression of BAX. Bax has been shown to be involved in p53-mediated apoptosis.
Description
Bax Mouse Recombinant amino acid 38-171 produced in E.Coli is a single, non-glycosylated polypeptide chain. The Mouse Bax is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The protein contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
References
1.Title:Bax Regulates Production of Superoxide in Both Apoptotic and Nonapoptotic Neurons: Role of Caspases.
Publication:The Journal of Neuroscience, 1 December 2010, 30(48): 16114-16127; doi: 10.1523/?JNEUROSCI.2862-10.2010 The Journal of Neuroscience, 1 December 2010, 30(48): 16114-16127; doi: 10.1523/?JNEUROSCI.2862-10.2010
Link:http://www.jneurosci.org/content/30/48/16114.full.pdf
2.Title:Interaction of IGF-binding Protein-3 and BAX in Mitochondria Promotes Male Germ Cell Apoptosis
Publication:First Published on November 3, 2009, doi: 10.1074/jbc.M109.046847 January 15, 2010 The Journal of Biological Chemistry, 285, 1726-1732.
Link:http://www.jbc.org/content/285/3/1726.full
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
