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第12年
- 入驻时间： 2012-04-17
- 联系人：客户经理
- 电话：400-968-7988
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联系时，请说明易展网看到的
- Email：fuwu@bioleaf.com
产品详情
简单介绍：
BID Mouse, GST
详情介绍：
Catalogue number
PRO-643
Synonyms
BH3-interacting domain death agonist, p22 BID, BID, FP497, MGC15319, MGC42355.
Introduction
BID is a pro-apoptotic Bcl-2 protein having only the BH3 domain. In reaction to apoptotic signaling, BID interacts with another Bcl-2 family of cell death regulators, called Bax, they form a heterodimer resulting to the insertion of Bax into the outer mitochondrial membrane. Bax induces the opening of the mitochondrial voltage-dependent anion channel which lead to the release of cytochrome c and other pro-apoptotic factors from the mitochondria resulting in activation of caspases. BID is a mediator of mitochondrial damage induced by caspase-8 (CASP8). CASP8 cleaves BID, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. The major proteolytic product p15 BID releasea cytochrome c. Isoform 1, Isoform 2 and Isoform 4 induce ice-like proteases and apoptosis while Isoform 3 does not induce apoptosis.
Description
BID Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 1-195 amino acids and having a molecular mass of 48 kDa.
The Mouse BID is expressed as GST-Tag fusion protein and purified by proprietary chromatographic technique.
The Mouse BID is expressed as GST-Tag fusion protein and purified by proprietary chromatographic technique.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless liquid formulation.
Formulation
The Mouse GST tag BID protein solution contains 10mM Tris-HCl pH-8, 1mM EDTA and 250mM NaCl.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Safety Data Sheet
SDS
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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