- 入驻时间: 2012-04-17
- 联系人:客户经理
- 电话:400-968-7988
-
联系时,请说明易展网看到的
- Email:fuwu@bioleaf.com
产品介绍 |
|||
品名 |
MicroVue Complement Fragment Panel (C4a,Ba, Bb, C3a, C5a, Sc5b-9, FH, FI) |
||
货号 |
A900 |
||
规格 | 1 Kit (96 well) | ||
格式 |
ELISA |
||
样品类型 |
Plasma/Serum |
||
检测时间 |
3 hours |
||
样品体积 |
50 μL (consult the Package Insert for dilution instructions for all sample types) |
||
使用说明 |
For Research Use Only. Not for use in diagnostic procedures. |
||
产品描述 |
The MicroVue Complement Multiplex assays measures the amount of various complement proteins in human plasma or serum specimens. Choose between available off-the-shelf assays or have a custom kit developed with the specific analytes required (see Ordering Information below for more information). The included analytes are available in two panels:
Why two panels?
|
||
兼容性 |
Certain analytes cannot be combined into the same custom multiplex assays due to cross-reactivity. Incompatible analytes include:
|
||
背景 |
The Complement System consists of more than 50 fluid and membrane-bound proteins and protein fragments organized into four primary pathways: the Classical, Alternative, Lectin and Terminal. Through specific activation steps complement proteins mediate a set of activities including inflammation, clearance of immune complexes, disruption of cell membranes, and regulation of the immune response. Deficiencies in the complement cascade can predispose individuals to infection through ineffective opsonization or defects in the ability to lyse invading pathogens. Also, the complement system is associated with many acute and chronic diseases due to abnormalities in its function. Quantitative analysis of different complement proteins and their activation products are of great importance in experimental and clinical medicine. The ability to measure multiple complement proteins in an individual sample constitutes a major step forward in research-based tools. The MicroVue Complement Multiplex can quantitively measure twelve individual complement proteins, providing a comprehensive overview of complement system homeostasis and activation. |
||
分析步骤 |
Sample volume per well (50 μL) Sample Dilution Antigen incubation time (2 hours) Wash wells (3X) Detection volume per well (50 μL) Detection incubation time (1 hour) Wash wells (3X) SHRP 1X volume per well (50 μL) SHRP 1X incubation time (20 minutes) Wash wells (6X) Substrate volume per well (50 μL) Imaging Time (250-300 sec) |