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- Email:fuwu@bioleaf.com
产品介绍 |
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品名 |
Monoclonal anti parvalbumin |
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货号 |
235 |
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规格 |
200 µl、500 µl 、1 ml |
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克隆性 |
monoclonal |
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共轭 |
nonconjugated |
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反应性 |
zebra finch, human, mouse, rat, zebrafish , domestic sheep, rhesus macaque |
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应用 |
immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section |
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宿主动物 |
Mouse monoclonal, IgG1 |
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抗体形式 |
Conc. supernat. lyophil. |
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产品形式 |
Lyophilized ascites (no preservatives). |
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复溶 |
with 200 µl bidistilled water. |
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产品描述 |
Monoclonal anti-Parvalbumin is a mouse IgG1 produced by hybridization of mouse myeloma cells with spleen cells from mice immunized with parvalbumin purified from carp muscles (1). The antibody was evaluated for specificity and potency: a) by indirect immunofluorescent or immunoperoxidase labeling as well as Avidin-Biotin staining of cryostat or vibratome-sections of 4% paraformaldehyde fixed tissue; b) by immunoenzymatic labelling of immunoblots; c) by radioimmunoassay (RIA)(1). The product is a monoclonal antibody (McAB) against parvalbumin, a calcium-binding protein of the EF-hand family related to calmodulin and troponin-C. The antibody reacts specifically with parvalbumin in tissue originating from human, monkey, rabbit, rat, mouse chicken and fish. The McAB specifically stains the 45Ca-binding spot of parvalbumin (MW 12'000 and IEF 4.9) in a two-dimensional "immunoblot". In a RIA set up the McAB measures parvalbumin with a sensitivity of 10 ng/assay and an affinity of 7.9 x 1012 L/M (1). |
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稀释 |
Immunohistochemistry: 1:5'000 - 1:10'000 Immunoblotting: 1:1'000. We recommend that the optimal dilution be determined by titration test.
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储存 |
For long storage, keep small aliquots of 1-2 µl at - 80°C (or at least - 20°C). For continous use keep diluted solutions at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing |
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参考文献 |
1. Celio M.R. et al., Cell Calcium 9:81-86, 1988 2. Celio M.R., Heizmann C.W. (1981) Nature 293: 300-302 3. Celio M.R., Heizmann C.W. (1982) Nature 297:504-506 4. Celio M.R. (1986) Science 231:995-997 5. Schwaller B., et al. (1999) Am. J. Physiol. 276. C395-403
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