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  • 产品名称:Monoclonal anti-Na+-Ca2+-exchanger

  • 产品型号:R3F1
  • 产品厂商:Swant
  • 产品价格:0
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简单介绍:
品牌:Swant 货号:R3F1 品名:Monoclonal anti-Na+-Ca2+-exchanger 规格:200ul
详情介绍:

产品介绍

品名

 Monoclonal anti-Na+ -Ca2+-exchanger 

货号

 R3F1

规格

 200ul

克隆性 

 monoclonal

物种反应

human, mouse, rat, sheep 

应用

 western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section, western blot knockout validation

宿主动物

 Mouse monoclonal, IgG1

抗体形式

 Conc. Supernat lyophil.

产品形式

 Lyophilized, concentrated supernatant.

复溶

 with 200 µl of bidistilled water.

产品描述

The monoclonal antibody against the canine Na+ -Ca2+-exchanger, recognizes two neighboring but non-overlapping sequences of the hydrophilic region of the exchanger, connecting the putative transmembrane segments 5 and 6. The antibody was evaluated for specificity and potency by immunoblotting, and immunoprecipitation (see reference). It recognizes virtually only NCX1 in monkey, horse, dog, rat, bovine and mouse tissue. At higher concentrations it reacts faintly with NCX2, whereas NCX3 is not recognized. Both splice variants with exon A (heart, nervous system) and exon B (kidney, glia) are recognized.

稀释

Immunohistochemistry: 1: 500; 

1: 1’000 Immunoblots and immunoprecipitation: 1: 500; 1: 1'000 (use milk for blocking). A strong 70 kDa band is found in addition to the 116 kDa of the integral exchanger. Often the HMW band is a double band 116/ 112 kDa band. 

The titer was determined using the indirect-peroxidase method. We recommend that the optimal dilutions be determined by titration experiments.

储存

After reconstitution freeze in small aliquots (e.g. 1 µl) and keep at - 80°C (or at least - 20°C). For continuous use keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.

参考文献

 Porzig H., Li Z., Nicoll D.A., Philipson K.D. (1993) Mapping the cardiac sodium-calcium exchanger with monoclonal antibodies. Am. J. Physiol. 265:C78 -C756, 1993

 

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