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产品介绍 |
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品名 |
Monoclonal antibody against human Insulin-like growth factor 2 |
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货号 |
73 |
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规格 |
200 µl |
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克隆性 |
monoclonal |
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宿主动物 |
Mouse monoclonal, IgG1 |
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抗体形式 |
Conc. supernat. lyophil. |
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产品形式 |
lyophilized concentrated supernatant (no preservatives). |
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复融 |
with 200 µl bidistilled water |
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产品描述 |
Antibody 73 is a mouse IgG1 directed against an epitope overlapping with residues 27-45 of IGF2. It has an apparent affinity constant of 5.5 l/nMol and can be used at a dilution of approx. 1:10’000 for RIA of human serum. A half maximal displacement is achieved by 1.2- 1.3 ng IGF2 per 0.2 ml. The lowest concentration detectable is 0.3 ng IGF2 per 0.2 ml. No crossreactivity is observed with human insulin (50 g/200 l), proinsulin (1 µg/200 µl) and D-peptide of IGF2 (10µg/200µl). Antibody 73 recognizes IGF 2 of other species with following approximate relative potencies: guinea pig, human, mouse, rabbit, rat = 1.3:1:<0.4:<0.1:<0.1. Other applications have not been tested. |
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稀释 |
For RIA of human serum: 1:10'000. For other applications: not tested. |
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储存 |
For long storage, keep small portions (1-2 µl) at -80° C (or at least at -20°C). For continuous use keep at 4°C (with 0.01 % Na-azide) Avoid repeated freezing and thawing. |
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参考文献 |
Läubli U.K. et al. (1982) Monoclonal antibodies directed to human insulin-like growth factor I (IGF I). FEBS Letters 149: 109-112 |