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Product Details | |
Product Name | Anti-Acetyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [RM149] |
Product Description | Rabbit monoclonal to Acetylated Histone H3 Lysine 4 (K4ac), H3K4ac, Histone H3 (K4 acetylation) |
Catalog No. | 31-1063-00 |
Clone Name | RM149 |
Product Data Sheet | Anti-Acetyl-Histone H3 Lys4 Rabbit Monoclonal Antibody Data Sheet RM149 |
Specificity | RM149 reacts to Histone H3 acetylated at Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in histone H3. |
Immunogen | An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys4). |
Application |
Western Blot (WB) Immunocytochemistry (ICC) ELISA Multiplex |
Species Reactivity |
Human Vertebrates |
Conjugate | None |
Intended Use | Research Use Only |
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Properties | |
Form | Liquid |
Storage Instructions | Store at -20.0°C |
Stability | Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Volume/Size | 100 ug |
Concentration | 1.0 mg/mL |
Purity | Protein A affinity purified from an animal origin–free culture supernatant |
Clone Type | Monoclonal |
Isotype | Rabbit IgG |
Usage |
Western Blot: 0.25 ug/mL - 1 ug/mL; ICC: 0.5 ug/mL - 2 ug/mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.05 ug/mL – 0.5 ug/mL. |
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Product Images | |
RM149 specifically reacts to Histone H3 acetylated |
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Western Blot of acid extracts from HeLa cells |
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Immunocytochemistry of HeLa cells treated with |