Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody, Clone RM129 32-1031-02 进口试剂采购网

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产品名称：Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody, Clone RM129
产品型号：32-1031-02
产品厂商：RevMAb
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Product Details
Product Name	Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody [RM129]
Product Description	Biotinylated Rabbit monoclonal to Human Immunoglobulin Light Chains
Catalog No.	32-1031-02
Clone Name	RM129
Product Data Sheet	Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody Data Sheet RM129
Specificity	RM129 reacts to both kappa and lambda light chains of human immunoglobulins. The antibody does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Immunogen	Human IgG
Application	Immunohistochemistry (IHC) Flow Cytometry (FC) Immunocytochemistry (ICC) ELISA
Species Reactivity	Human
Conjugate	Biotin
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size	50ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	ELISA: 0.02ug/mL – 0.25ug/mL; Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL; Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
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Product Images
	Detection of human IgG1 in monkey serum, using RM117 (capture) and biotin-RM129 (detection) as a Sandwich ELISA pair. HRP conjugated streptavidin and TMB were used to yield the colorimetric reaction.
	ELISA showing RM129 reacts only to kappa and lambda light chain of all human immunoglobulins, not to mouse IgG, rat IgG, or goat IgG.
	ELISA showing RM129 reacts only to kappa and lambda light chain of all human immunoglobulins, not to mouse IgG, rat IgG, or goat IgG.
	ELISA showing RM129 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM129 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
	A titer Sandwich ELISA using Biotinylated RM129 as the detection antibody. The plate, coated with the capture antibody anti-human IgG RM116, was loaded with different amounts of human IgG. A serial dilution of Biotin RM129 was used as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.