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  • 产品名称:Monoclonal anti Calbindin D-28k 1ml

  • 产品型号:300
  • 产品厂商:Swant
  • 产品价格:0
  • 折扣价格:0
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简单介绍:
品牌:Swant 货号:300 品名:Monoclonal anti Calbindin D-28k 规格:1 ml
详情介绍:

产品介绍

品名

Monoclonal anti Calbindin D-28k 

货号

300

规格

200ul / 500ul  / 1ml

 克隆性

monoclonal

共轭

nonconjugated

反应性

thirteen-lined ground squirrel, Spanish newt, slender lungfish, reedfish , Shaw's jird, gray bichir , African green monkey, human, mouse, rat, chicken, pigs , Xenopus laevis, rhesus macaque

应用

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section

宿主动物

Mouse monoclonal, IgG1

抗体形式

Conc. supernat. lyophil.

品形式

Lyophilized concentrated supernatant (no preservatives). 

复溶

with 200 µl / 500 µl or 1 ml bidistilled water

产品描述

Monoclonal anti-calbindin D-28k is a mouse IgG1 produced by hybridization of mouse myeloma cells with spleen cells from mice immunized with calbindin D-28k purified from chicken gut (1). The antibody was evaluated for specificity and potency: a) by indirect immunofluorescent or immunoperoxidase labelling as well as Biotin-Avidin labeling of cryostate or vibratome-sections of 4 % paraformaldehyde fixed tissue; b) by immunoenzymatic labelling of immunoblots; c) by radioimmunoassay. 

The product is a monoclonal antibody (McAB) against calbindin D-28k, a calcium-binding protein of the EF-hand family related to calmodulin and troponin-C

稀释

Immunohistochemistry: 1:5'000 - 1:10'000 with the avidin-biotin method.

Immunoblots: 1:100 - 1:1'000 with an indirect peroxidase method. 

We recommend that the optimal dilutions be determined by titration experiments.

储存

For long storage, keep at - 80°C (or at least - 20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing. 

参考文献

1. Celio M.R. et al., Cell Calcium 11:599-602, 1990 

2. Kretsinger R.H. (1981) Neurosci. Res. Progr. Bull. 19/8, MIT-Press 

3. Garcia-Segura L.M. et al. (1984) Brain Rews. 296: 75-86. 

4. Airaksinen M.S., et al, (1997), PNAS 94(4) : 1488-1493

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