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产品介绍 |
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品名 |
Affinity purified mouse monoclonal anti Calbindin D-28k |
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货号 |
300PUR |
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规格 |
100 µl |
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形式 |
Purified antibody in PBS (lyophilized). |
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复溶 |
with 100 µl bi-distilled water. |
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描述 |
The antibody 300pur was purified with Protein-G from in vitro produced monoclonal antibody 300 directed against calbindin D-28k (see product description 300 for further details concerning immunoblot and immunohistochemistry). The antibody concentration is of 0.39 mg/ml in 0.1M phosphate buffered saline, pH 7.3. |
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稀释 |
We suggest using a dilution of 1:10’000 (0.039 g/ml) for immunostaining with the avidin-biotin method on cryostat or paraffin-sections of paraformaldehyde (4%) or formalin-fixed tissue. We recommend that the optimal dilutions be determined by titration experiments. |
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储存 |
For long storage, keep at - 80°C (or at least - 20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing |
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参考文献 |
1. Celio M.R. et al., Cell Calcium 11:599-602, 1990 2. Kretsinger R.H. (1981) Neurosci. Res. Progr. Bull. 19/8, MIT-Press 3. Garcia-Segura L.M. et al. (1984) Brain Res. 296: 75-86. 4. Airaksinen M.S., et al, (1997), PNAS 94(4) : 1488-1493 |