Corning® Fibronectin, human, 5mg, is used as a thin coating on tissue-culture surfaces to promote attachment, spreading, and proliferation of a variety of cell types. The principal functions of fibronectin appear to be in cellular migration during wound healing and development, regulation of cell growth and differentiation, and haemostasis/thrombosis.
Quality
Purity = 90% by SDS-PAGE
Tested for ability to promote attachment and spreading of BHK-21 cells
Found negative for bacteria, fungi, and mycoplasma
Source
Human plasma. Source material tested for hepatitis B antigen and HIV-1 antibody.
Molecular Weight
440 kD (unreduced form)
Preparation and Storage
Stable for three months at 2° to 8°C (lyophilized) or two weeks at -20°C (solubilized)
Handling
Recommended concentration - 1-5 µg/cm² of growth surface for coating or at 5 µg/ml as a media additive
Formulation
Lyophilized from CAPS buffer containing NaCl and CaCl2, pH 11.0
