Lyophilized enzyme and affinity resin for above hinge digestion of human IgG1 and purification of Fab fragments.
GingisKHAN Fab Kit contains one vial of lyophilized GingisKHAN, five vials of GingisKHAN Reducing Agent for antibody digestion, and four microspin columns of CaptureSelect™* CH1 resin for affinity purification of the Fab fragments. The CaptureSelect™ IgG-CH1 affinity matrix recognizes the CH1 domain of human IgG antibodies, enabling purification of Fab fragments independent on the light-chain isotype. Due to its unique selectivity for the CH1 domain, no co-purification of free light-chain contaminants will occur. Each CaptureSelect™ microspin column contains sufficient material to purify 0.5 mg human IgG1.
•Purification of homogeneous Fab fragments from human IgG1
•Reliable workflow for preparative applications
•Enables chain pairing characterization of bispecific antibodies
