OmniGLYZOR is used for deglycosylation of glycoproteins carrying N- and simple O-glycans.
OmniGLYZOR Kit consists of:
•OmniGlyZOR Microspin columns
•PNGase F Lyophilized
•RapiGest™* SF surfactant
Certain N-glycosylation sites are poorly or not accessible to PNGase F unless the substrate protein is denatured. Remaining N-glycans after incubation on the OmniGLYZOR Microspin column can be removed by an additional deglycosylation step under denaturing conditions using the PNGase F Lyophilized and RapiGest™ SF surfactant included in the kit.
The enzymes hydrolyzing the O-glycans do not benefit from denaturation which is why the OmniGLYZOR columns should only be used under native conditions.
OmniGLYZOR Kit Microspin is provided in kits containing sufficient material to deglycosylate 5 × 50-100 µg or 10 × 50-100 µg glycoprotein.
•Fast and efficient one-pot deglycosylation of N- and O-linked glycans
•Compatible with LC-MS
•Complete removal of glycans for improved analysis of underlying protein
•Immobilized format increases enzyme-to-substrate ratio to enable fast deglycosylation
