Immobilized enzyme for on-column digestion of IgG below the hinge. FabRICATOR (IdeS) is an IgG-specific cysteine protease that digests antibodies at a single amino acid site below the hinge, generating homogenous F(ab’)2 and Fc fragments.
After digestion, the fragments can be analyzed using reversed-phase HPLC, mass spectrometry or other analytical method. Automation of antibody digestion and subunit generation enables applications in online monitoring of bioreactors, for example as part of a 2D-LC-MS setup, coupling the reactor directly to the MS. This automated approach of antibody subunit analysis reduces operator time, sample handling and increases the throughput.
•Automated and consistent middle-level workflows
•Robust on-column enzyme performance
•Easy to integrate into multidimensional LC setups
