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- 产品名称：活体单细胞毒性荧光检测试剂盒,Live, Single Cell-based Fluorogenic Cytotoxicity Assay Assay Kit
- 产品型号：CyToxiLux® , GranToxiLux®, and PanToxiLux™
- 产品展商：phiphilux
- 产品文档：无相关文档
- 发布时间：2018-06-23
- 在线询价
简单介绍
CyToxiLux® , GranToxiLux®, and PanToxiLux™
Live, Single Cell-based Fluorogenic Cytotoxicity Assay Assay Kits
A tumor cell is engaged and then "killed" by a cytotoxic lymphocyte
Principle of assays:
Following the successful delivery of a lethal hit by cytotoxic lymphocytes, protease activities leading to cell death in individual target cells can be measured by flow cytometry or fluorescence mi
产品描述
CyToxiLux® , GranToxiLux®, and PanToxiLux™
Live, Single Cell-based Fluorogenic Cytotoxicity Assay Assay Kits
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A tumor cell is engaged and then "killed" by a cytotoxic lymphocyte
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Principle of assays:
Following the successful delivery of a lethal hit by cytotoxic lymphocytes, protease activities leading to cell death in individual target cells can be measured by flow cytometry or fluorescence microscopy. In each kit cleavage of a cell permeable fluorogenic substrate reports the following activities:
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CyToxiLux® : downstream caspase
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GranToxiLux®: granzyme B
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PanToxiLux™: granzyme B and upstream caspase
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Measurements with CyToxiLux®, GranToxiLux®, and PanToxiLuxTM are superior to bulk assays e.g., LDH and 51Cr release, in terms of both time (0.3-2.0 hr. vs. 4 hr.) and sensitivity (relatively weak CTL responses against subdominant epitopes are detectable).
Detailed CyToxiLux® PLUS! instructions are available for download in PDF format
Detailed GranToxiLux® PLUS! instructions are available for download in PDF format
Detailed PanToxiLux® instructions are available for download in PDF format
Detailed PanToxiLux™ P2D2 instructions are available for download in PDF format
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