RID读取器是一种经济高效且紧凑的仪器,旨在简化和加速读取沉淀素径向**扩散环的过程。
这款便携式仪器针对使用RID板的所有规模的实验室,通过*少的用户输入就可以有效地定量已知蛋白质的浓度,从而减少了用户错误。
RID阅读器具有一个内置摄像机,可以将沉淀环的大而清晰的图像传输到计算机屏幕上,以提高测量精度。
直观的软件使用户可以从多个选项中进行选择,以根据自动生成的校准曲线计算结果,从而提高对结果的信心。
可以保存数据,校准曲线和图像以备将来参考,从而为培训和质量控制提供了有用的工具。
该技术基于抗原和抗体的复合以产生可见的沉淀素环。沉淀素环的直径与样品中蛋白质的含量成正比,可以使用目镜进行测量,或者使用Binding Sites Digital RID Plate Reader进行更**的测量。
我们的大多数RID分析均基于常规抗体-抗原技术。在需要提高灵敏度的地方,我们开发了NANORID ™技术。这些试剂盒使用乳胶增强抗体,即使在低蛋白质浓度下也能进行准确定量。
套件功能
多种方法:我们所有的RID分析均包含执行三种不同分析方法的程序:
RID参考表(Mancini)
完全扩散(曼奇尼)
不完全扩散(Fahey和McKelvey)
具有成本效益: RID板可以分段以允许每个板使用不止一次
更高的精度和精密度:提供了样品稀释剂以减少样品之间的粘度差异
带有视频摄像头技术的放大和清晰定义的戒指显示在计算机屏幕上
轻松定量环尺寸可提高结果准确性
*少的维护和快速的校准,再加上简单的软件,使得数字RID读板机非常人性化
明亮的内部光源
实现一致且恒定的查看以进行**测量
RID板保持在阅读器中
与更传统的阅读方法相比,稳定观看戒指可减少用户出错的机会
校准曲线由软件绘制并自动计算结果
提高实验室效率并消除转录错误的机会
所有数据和图像均可存储
无需笨重的纸质文件,并且可以快速/轻松地访问所有数据
数字RID技术规范
LED阵列光源
尺寸:220mm(W)140mm(H)160mm(D); 8.66英寸(宽)x 5.51英寸(高)x 6.30英寸(深)
仪器重量:1.14 kg;2.51磅
CCR视频系统:628 x 582像素
计算机要求:USB端口时,Windows ® XP 2000,XP,Vista和Windows ® 7
软件
径向**扩散方法各不相同,并且可以几种不同方式计算结果。RIDRead软件包的灵活性允许用户从校准曲线中选择其**的结果计算方法:
如果精度至关重要,则使用线性曲线
如果需要快速求出结果,请选择二项式曲线(使用Fahey方法)
点对点曲线用于功能补体测定
The RID Reader is a cost effective & compact instrument, designed to simplify & speed-up the process of reading precipitin radial immunodiffusion rings.
This portable instrument is aimed at all sizes of laboratories using RID plates, decreasing user error by efficiently quantifying the concentrations of known proteins with minimal user input.
The RID Reader has a built-in video camera that transmits large, clear images of the precipitin rings to the computer screen to improve measurement precision.
The intuitive software enables the user to select from several options for the calculation of results from an automatically generated calibration curve to increase confidence in results.
Data, calibration curves and images can be saved for future reference, providing a useful tool for training and quality control.
The technique is based on the complexing of antigen and antibody to produce a visible precipitin ring. The diameter of the precipitin ring is directly proportional to the amount of protein present in the sample and can be measured using an eye piece or, for more precise measurement, with Binding Sites Digital RID Plate Reader.
The majority of our RID assays are based on conventional antibody-antigen technology. Where increased sensitivity is required, we have developed NANORID™ technology. These kits use latex enhanced antibodies to give accurate quantitation, even at low concentrations of protein.
Kit Features
Multiple Methods: All of our RID assays contain the procedures for performing three different assay methods:
RID reference table (Mancini)
Complete diffusion (Mancini)
Incomplete diffusion (Fahey and McKelvey)
Cost efficient: RID plates can be sectioned to allow each plate to be used more than once
Improved Accuracy and Precision: A sample diluent is provided to reduce viscosity differences between samples
Enlarged & clearly defined rings are displayed on the computer screen with video camera technology
Easy quantification of ring size increases accuracy of results
Minimal maintenance and quick calibration combined with simple software make the digital RID plate reader very user friendly
Bright internal light source
Enables consistent & constant viewing for precise measurement
RID plate is kept still in the reader
Steady viewing of the rings decreases chances of user error compared to more traditional reading methods
Calibration curves are drawn by the software & results are calculated automatically
Increases lab efficiency & negates the chance of transcription errors
All data & images can be stored
No need for bulky paper files & all data can be quickly / easily accessed
Digital RID technical specifications
LED array light source
Dimensions: 220mm (W) 140mm (H) 160mm (D); 8.66in (W) x 5.51in (H) x 6.30in (D)
Instrument Weight: 1.14 kg; 2.51 lbs
CCR video system: 628 x 582 pixels
Computer Requirements: USB Port, Windows® XP 2000, XP, Vista & Windows® 7
Software
Radial immunodiffusion methods vary and results can be calculated in several different ways. The flexibility of the RIDRead package allows the user to select their preferred option for result calculation from a calibration curve:
A linear curve is used if accuracy is of primary importance
Choose a binomial curve if results are required quickly (uses a Fahey method)
A point-to-point curve is used for functional complement assays
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