基本信息
货号：12500 (1mg/1mL)
储存方式：保持在-20 oC。到期日为自收到之日起一年。
CAS号：61970-00-1
分子量：~60kDa
形式：液体（1mg / mL）
测量范围：≤1飞摩尔ATP敏感度（使用0.2μg荧光素酶和适当敏感的液体闪烁计数器或发光计）
生物应用
该重组萤火虫荧光素酶是从来自北美萤火虫（Photinus pyralis）的克隆基因表达的酶，其提供使用生物发光试剂检测ATP或荧光素底物进行研究或生产试剂盒所需的可靠性和可靠性。 该重组酶可以潜在地消除可能在从天然来源纯化的荧光素酶中发现的季节性和区域变异性的可能性。
该酶与荧光素，ATP和O2的反应导致光的发射。 荧光素酶可用于检测痕量的ATP。 萤火虫荧光素酶也是基因表达研究中*常用的报告基因之一。 荧光素酶催化的生物发光反应是测量基因表达的*灵敏的分析工具之一。 通过使用0.2μg荧光素酶可以检测小于或等于1飞摩尔的ATP。
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