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- 产品名称：MicroVue Serum PYD EIA
- 产品型号：8019
- 产品展商：Quidel
- 产品文档：无相关文档
- 发布时间：2016-12-30
- 在线询价
简单介绍
MicroVue Serum PYD provides a quantitative measure of the excretion of pyridinoline cross-link (Pyd) in serum. Pyridinoline cross-links are an indicator of type I collagen resorption, especially bone collagen.
产品描述
For Research Use Only in the United States.
Not for use in diagnostic procedures.
MicroVue Serum PYD provides a quantitative measure of the excretion of pyridinoline cross-link (Pyd) in serum. Pyridinoline cross-links are an indicator of type I collagen resorption, especially bone collagen.
In humans, the total pool of urinary Pyd is approximately 45% free while the remaining fraction is bound to oligopeptides ranging from small linear peptides to very large cross-linked structures in excess of 10,000 Da. The proportion of free to total cross-links appears to be constant in healthy individuals and those with metabolic bone diseases or arthritis conditions, thus providing the rationale for measuring free Pyd.
Format
96 well microtiter plate, with reagents sufficient to test 40 samples in duplicate
Antibody Specificity
Free pyridinoline cross-links, some cross-reactivity to glutamine and arginine (0.5 nmol/L signal)
Species Reactivity
Baboon, Cat, Cow, Dog, Guinea Pig, Horse, Human, Rhesus Macaque, Mouse, Rat
No reactivity detected with Rabbit
Assay Steps
Spin serum in Spinfilters (included) for 30 minutes
Add 50 µL Reagent 1 to plate
Add 25 µL serum, diluted Standards, diluted Controls
Add 75 µL cold Pyridinoline Antibody
Incubate overnight at 2°C to 8°C
Wash 3 times with 1x Wash Buffer
Add 150 µL Enzyme Conjugate
Incubate 1 hour at room temperature
Wash 3 times with 1x Wash Buffer
Add 150 µL working Substrate Solution
Incubate 40 +/– 5 minutes at room temperature
Add 100 µL Stop Solution
Read at 405 nm
Data Reduction: 4 Parameter
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