Description
DESCRIPTION
MW= 38.8 kDa. Full length nsp15 (endoRNAse) from SARS-CoV-2, expressed in E. coli. The protein consists in residues 1-346 (NCBI reference sequence YP_009725310.1).
SEQUENCE
Numeration of the nsp15 from NCBI YP_009725310.1
10 20 30 40 50 60
SLENVAFNVV NKGHFDGQQG EVPVSIINNT VYTKVDGVDV ELFENKTTLP VNVAFELWAK
70 80 90 100 110 120
RNIKPVPEVK ILNNLGVDIA ANTVIWDYKR DAPAHISTIG VCSMTDIAKK PTETICAPLT
130 140 150 160 170 180
VFFDGRVDGQ VDLFRNARNG VLITEGSVKG LQPSVGPKQA SLNGVTLIGE AVKTQFNYYK
190 200 210 220 230 240
KVDGVVQQLP ETYFTQSRNL QEFKPRSQME IDFLELAMDE FIERYKLEGY AFEHIVYGDF
250 260 270 280 290 300
SHSQLGGLHL LIGLAKRFKE SPFELEDFIP MDSTVKNYFI TDAQTGSSKC VCSVIDLLLD
310 320 330 340
DFVEIIKSQD LSVVSKVVKV TIDYTEISFM LWCKDGHVET FYPKLQ
ISOTOPIC ENRICHMENT
none
SUPPLIED AS
Protein solution. The concentration is calculated from the absorbance at 280nm (ε280 = 33140 M-1cm-1).
STORAGE
-20°C. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.
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