Description
DESCRIPTION
MW= 33.3 kDa. Full length nsp16 (2′-O-ribose methyltransferase) from SARS-CoV-2, expressed in E. coli. The protein consists in residues 1-298 (NCBI reference sequence YP_009725311.1).
SEQUENCE
Numeration of the nsp16 from NCBI YP_009725311.1
10 20 30 40 50 60
SSQAWQPGVA MPNLYKMQRM LLEKCDLQNY GDSATLPKGI MMNVAKYTQL CQYLNTLTLA
70 80 90 100 110 120
VPYNMRVIHF GAGSDKGVAP GTAVLRQWLP TGTLLVDSDL NDFVSDADST LIGDCATVHT
130 140 150 160 170 180
ANKWDLIISD MYDPKTKNVT KENDSKEGFF TYICGFIQQK LALGGSVAIK ITEHSWNADL
190 200 210 220 230 240
YKLMGHFAWW TAFVTNVNAS SSEAFLIGCN YLGKPREQID GYVMHANYIF WRNTNPIQLS
250 260 270 280 290
SYSLFDMSKF PLKLRGTAVM SLKEGQINDM ILSLLSKGRL IIRENNRVVI SSDVLVNN
ISOTOPIC ENRICHMENT
none
SUPPLIED AS
Protein solution. The concentration is calculated from the absorbance at 280nm (ε280 = 56630 M-1cm-1).
STORAGE
-20°C. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.
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