SOD1 Cu/Zn > SOD1 Cu/Zn - no lab[产品打印页面]

Description
MW = 32.6 kDa (dimer) calculated, 32.9 kDa (metallated dimer). Human WT-SOD1 cloned from Human cDNA, expressed in E. coli. The protein consists of the full length human Cu, Zn SOD1 S-S (residues 1-154, UniProtKB accession P00441). Four additional residues, GSFT, are present at the N-term of the monomers.

Sequence

             10         20         30         40         50
GSFT-MATKAVCVLK GDGPVQGIIN FEQKESNGPV KVWGSIKGLT EGLHGFHVHE 
             60         70         80         90        100
     FGDNTAGCTS AGPHFNPLSR KHGGPKDEER HVGDLGNVTA DKDGVADVSI 
            110        120        130        140        150
     EDSVISLSGD HCIIGRTLVV HEKADDLGKG GNEESTKTGN AGSRLACGVI 

     GIAQ

 

Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as single band (monomer) migrating at a molecular weight between 14.4 and 18.4 kDa. The Cu,Zn SOD1 S-S is stable as dimer. The metallation state is verified by NMR and/or mass analysis.

Storage
-20°C. The protein is stable at 4°C for at least 2 weeks and at 25°C for several hours. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.