Description
Description
MW = 17.6 kDa calculated. Recombinant Matrix Metalloproteinase-8 (MMP-8, Collagenase-2, Neutrophil collagenase) cloned from human cDNA, expressed in E. coli. The enzyme consists of the catalytic domain of human MMP-8 (residues 105-262, UniProtKB accession P22894).
Sequence
110 120 130 140
NPKWER TNLTYRIRNY TPQLSEAEVE RAIKDAFELW
150 160 170 180
SVASPLIFTR ISQGEADINI AFYQRDHGDN SPFDGPNGIL
190 200 210 220
AHAFQPGQGI GGDAHFDAEE TWTNTSANYN LFLVAAHEFG
230 240 250 260
HSLGLAHSSD PGALMYPNYA FRETSNYSLP QDDIDGIQAI
YG
Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at a molecular weight between 14.4 and 18.4 kDa.
Supplied as
0.2 mg/mL solution in Tris 20 mM pH 7.2, CaCl
2 10 mM, ZnCl
2 0.1 mM, NaCl 0.3 M, acetohydroxamic acid (AHA) 0.5 M. The concentration is calculated by the analysis of the absorbance at 280 nm (ε
280 = 28420 M
-1cm
-1 calculated).
Specific activity
> 80 U/μg. Activity described as U=100 pmol/min at 25°C using a colorimetric ***** with thiopeptide Ac-Pro-Leu-Gly-[2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5 (Biomol) as substrate.
Storage
-80°C. After initial defrost, aliquot the product into individual tubes and refreeze at -80°C.
Avoid repeated freeze/thaw cycles.
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