Sequence
10 20 30 40 50
MMCSSLEQAL AVLVTTFHKY SCQEGDKFKL SKGEMKELLH KELPSFVGEK
60 70 80 90
VDEEGLKKLM GSLDENSDQQ VDFQEYAVFL ALITVMCNDF FQGCPDRP
Purity
> 95% by SDS-PAGE. The protein is observed, in denaturating conditions, as two bands representing the monomer, migrating at a molecular weight below 14.4 kDa, and the dimer, migrating at a molecular weight between 18.4 and 25.0 kDa.
Supplied as
1 mg/mL solution in Tris 50 mM, pH 7.5, CaCl2 5 mM, DTT 2 mM. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 2980 M-1cm-1 calculated).
Storage
-20°C. The protein is stable at 4°C for at least 2 weeks and at 25°C for several hours. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.