Sequence
10 20 30 40 50
MTCKMSQLER NIETIINTFH QYSVKLGHPD TLNQGEFKEL VRKDLQNFLK
60 70 80 90 100
KENKNEKVIE HIMEDLDTNA DKQLSFEEFI MLMARLTWAS HEKMHEGDEG
110
PGHHHKPGLG EGTP
Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as single band (monomer) migrating at a molecular weight below 14.4 kDa.
Supplied as
1 mg/mL solution in Tris 10 mM pH 7.5, CaCl2 1 mM, NaCl 100 mM. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280= 6990 M-1cm-1 calculated).
Storage
-20°C. The protein is stable at 4°C for at least 2 weeks and at 25°C for at least several hours. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.