Sequence
10 20 30 40 50
MSDCYTELEK AVIVLVENFY KYVSKYSLVK NKISKSSFRE MLQKELNHML
60 70 80 90 100
SDTGNRKAAD KLIQNLDANH DGRISFDEYW TLIGGITGPI AKLIHEQEQQ
SSS
Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as single band (monomer) migrating at a molecular weight below 14.4 kDa.
Supplied as
1 mg/mL solution in MES 20 mM pH 5.5, KCl 200 mM, DTT 1 mM. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 12950 M-1cm-1 calculated).
Storage
-20°C. The protein is stable at 4°C for at least 2 weeks and at 25°C for several hours. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.