26S Proteasome Complex Subunit SEM1 (SEM1) siRNA abx933292 上海玉博生物科技有限公司

26S Proteasome Complex Subunit SEM1 (SEM1) siRNA

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产品名称：26S Proteasome Complex Subunit SEM1 (SEM1) siRNA
产品型号：abx933292
产品展商：abbexa
产品文档：无相关文档

简单介绍

26S Proteasome Complex Subunit SEM1 (SEM1) siRNA

产品描述

siRNA to inhibit SHFM1 expression using RNA interference.

This product is provided as three 5 nmol vials (15 nmol) or 2x three 5 nmol vials (30 nmol) of lyophilized siRNA oligo duplexes. Each vial contains slightly different sequences to ensure full knockout of the gene. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot.

Target

26S Proteasome Complex Subunit SEM1 (SEM1)

Reactivity

Human

Tested Applications

RNAi

Host

Synthetic

Recommended dilutions

Optimal dilutions/concentrations should be determined by the end user.

Plate (wells)	Final Medium Volume (ml)	Final siRNA Concentration (nM)	20 µM siRNA Volume (µl)	Lipofectamine 2000 Volume (µl)
96	0.1	100	0.5	0.25
		50	0.25	0.25
		10	0.05	0.25
24	0.5	100	2.5	1
		50	1.25	1
		10	0.25	1
12	1	100	5	2
		50	2.5	2
		10	0.5	2
6	2	100	10	5
		50	5	5
		10	1	5

Form

Lyophilized

Purity

> 97%

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Storage

Shipped at 4 °C. Store at -20 °C for up to one year.

UniProt Primary AC

P60896 (UniProt, ExPASy)

Gene Symbol

SHFM1

GeneID

7979

NCBI Accession

NM_006304.1

KEGG

hsa:7979

Specificity

SHFM1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Availability

Shipped within 7-15 working days.

Note

This product is for research use only.

Directions for use

Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube.

Resuspend the siRNA oligos to an appropriate concentration with DEPC water (e.g. resuspend one vial of 5 nmol siRNA oligo in 250 µl of DEPC water for a final concentration of 20 µM).

Transfect with 10 nM - 100 nM siRNA 48 to 72 hours prior to cell lysis.

Research Articles on 26S Proteasome Complex Subunit SEM1 (SEM1)

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26S Proteasome Complex Subunit SEM1 (SEM1) siRNA