Alpha-Methylacyl-CoA Racemase (AMACR) Antibody abx227135 上海玉博生物科技有限公司

Alpha-Methylacyl-CoA Racemase (AMACR) Antibody

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产品名称：Alpha-Methylacyl-CoA Racemase (AMACR) Antibody
产品型号：abx227135
产品展商：abbexa
产品文档：无相关文档

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Alpha-Methylacyl-CoA Racemase (AMACR) Antibody

产品描述

Alpha-Methylacyl-CoA Racemase (AMACR) Antibody is a Rabbit Monoclonal antibody for the detection of P504S (AMACR).

Target	Alpha-Methylacyl-CoA Racemase (AMACR)
Clonality	Monoclonal
Clone	P495
Reactivity	Human
Tested Applications	IHC
Host	Rabbit
Recommended dilutions	IHC-P: 1/100 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Synthetic peptide derived from the N-terminal region of human P504S.
Isotype	IgG
Form	Liquid
Purification	Purified from rabbit antiserum by proprietary techniques.
Storage	Store at 2-8°C.
UniProt Primary AC	Q9UHK6 (UniProt, ExPASy)
Buffer	20 mM Tris-HCl, pH 8.0, containing 20 mg/ml BSA and 0.05% NaN3.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.
Directions for use	Suggested IHC-P Protocol Preparation of Citrate Buffer (10 mM Citric Acid, 0.05% Tween-20, pH 6.0): Dissolve 1.92 g of anhydrous citric acid in 700 ml of distilled water. Adjust pH to 6.0 with 1 M NaOH and then add 0.5 ml of Tween-20 and mix thoroughly. Adjust the final volume to 1 L with distilled water. Store this solution at room temperature for up to 3 months or at 4 °C for long-term storage. Preparation of Wash Buffer: Use PBS, pH 7.0-7.5, containing 0.05% Tween-20. Deparaffinize the section in 3 changes of xylene, 10 minutes each. Wash the section in 96%, 80% and 70% ethanol, 10 minutes each. Rinse twice in distilled water, 5 minutes each. Block endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H₂O₂) for 10 minutes. Wash twice in distilled water, 5 minutes each. Antigen retrieval: immerse the slide in Citrate buffer, pH 6.0, and incubate in a water bath for 25 minutes at 95-97 °C. Remove the slide from the water bath and allow to stand at room temperature (in Citrate buffer, pH 6.0) for 15 minutes. Rinse twice in distilled water, 5 minutes each. Wash twice in Wash Buffer, 5 minutes each. Incubate the section with primary antibody at 1/100 - 1/200 dilution for 1 hour in a closed wet chamber. It is recommended to use abx291502 Primary Antibody Diluent or a diluent containing protease-free BSA (> 1 mg/ml) to dilute this antibody. Wash 3 times with Wash Buffer, 5 minutes each. Add the secondary antibody and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). It is recommended to use abx291501 Rabbit and Mouse HRP/DAB Detection Kit. Wash 3 times in Wash Buffer, 5 minutes each. Apply the DAB chromagen for 1-3 minutes. Wash twice in water, 5 minutes each. Stain in hematoxylin for 5 minutes. Wash 3 times in water, 2 minutes each. Mount the slide for observation.

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Alpha-Methylacyl-CoA Racemase (AMACR) Antibody