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The pGL4.12[luc2CP] Vector(a–d) encodes the luciferase reporter gene luc2CP (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been optimized for mammalian expression.
The pGL4.12[luc2CP] Vector is a basic vector with no promoter. However, it contains a multiple cloning region to allow cloning of a promoter of choice. The luc2CP reporter gene contains hCL1 and hPEST, both of which are protein destabilization sequences. The protein encoded by luc2CP responds more quickly and with greater a magnitude to changes in transcriptional activity than the luc2 gene, its more stable counterpart.