精氨酸酶(ARG)重组蛋白 ARG

精氨酸酶(ARG)重组蛋白

• 物种Homo sapiens (Human，人)相同的名称，不同的物种。
• 性状冻干粉内**水平<1.0EU/µg(LAL法测定)
• 亚细胞定位n/a
• 预测分子量36.3kDa
• 实际分子量-(差异分析请参阅说明书)
• 片段与标签Met1~Lys322 (Accession # P05089) with N-terminal His Tag
• 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
• 纯度> 95%
• 等电点-
• 应用SDS-PAGE; WB; ELISA; IP
  精氨酸酶(ARG)重组蛋白用法
  PBS或其它。
  储存
  避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
  稳定性
  热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
  
  精氨酸酶(ARG)重组蛋白
  Arginase (Arg)
  Organism: Homo sapiens (Human)
  Instruction manual
  FOR IN VITRO USE AND RESEARCH USE ONLY
  NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES
  4th Edition (Revised in August, 2012)
  精氨酸酶(ARG)重组蛋白[ DESCRIPTION ]
  Protein Names: Arginase
  Synonyms: Arg
  Species: Human
  Size: 100µg
  Source: Escherichia coli-derived
  Subcellular Location: Cytoplasm.
  精氨酸酶(ARG)重组蛋白[ PROPERTIES ]
  Residues: Met1~Lys322 (Accession # P05089), with N-terminal His-Tag.
  Grade & Purity: >95%, 36 kDa as determined by SDS-PAGE reducing conditions.
  Formulation: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.
  Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method). Applications: SDS-PAGE; WB; ELISA; IP.
  (May be suitable for use in other assays to be determined by the end user.)
  Predicted Molecular Mass: 36.3 kDa
  Predicted isoelectric point: 6.7
  [ PREPARATION ]
  Reconstitute in sterile PBS, pH7.2-pH7.4.[ STORAGE AND STABILITY ]
  Storage: Avoid repeated freeze/thaw cycles.
  Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
  Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was
  determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious
  degradation and precipitation were observed. (Referring from China Biological Products Standard, which was
  calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under
  appropriate storage condition.
  [ SEQUENCES ]
  The target protein is fused with N-terminal His-tag, its sequence is listed below.
  MGHHHHHHSGSEF-MSAKSRTIGI IGAPFSKGQP RGGVEEGPTV LRKAGLLEKL KEQECDVKDY GDLPFADIPN DSPFQIVKNP
  RSVGKASEQL AGKVAEVKKN GRISLVLGGD HSLAIGSISG HARVHPDLGV IWVDAHTDIN TPLTTTSGNL HGQPVSFLLK
  ELKGKIPDVP GFSWVTPCIS AKDIVYIGLR DVDPGEHYIL KTLGIKYFSM TEVDRLGIGK VMEETLSYLL GRKKRPIHLS
  FDVDGLDPSF TPATGTPVVG GLTYREGLYI TEEIYKTGLL SGLDIMEVNP SLGKTPEEVT RTVNTAVAIT LACFGLAREG
  NHKPIDYLNP PK