|
名称
|
货号
|
英文名、缩写
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分子量
|
规格
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价格
|
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DBCO-PEG-COOH
|
PS2-CMDB-5K
|
DBCO-PEG-COOH
|
5000
|
0.1g、0.5g
|
现询
|
|
DBCO-PEG-COOH
|
PS2-CMDB-2K
|
DBCO-PEG-COOH
|
2000
|
0.1g、0.5g
|
现询
|
|
DBCO-PEG-COOH
|
PS2-CMDB-3K
|
DBCO-PEG-COOH
|
3400
|
0.1g、0.5g
|
现询
|
|
DBCO-PEG-COOH
|
PS2-CMDB-1K
|
DBCO-PEG-COOH
|
1000
|
0.1g、0.5g
|
现询
|
|
DBCO-PEG-COOH
|
PS2-CMDB-10K
|
DBCO-PEG-COOH
|
10000
|
0.1g、0.5g
|
现询
|
（备注：非常规分子量：400、600。**聚合度的小分子定制。）
结构式：
基本信息：
中文名称：Dibenzocyclooctyne-聚乙二醇-羧基 (DBCO-PEG-COOH)
别名：DBCO-PEG-COOH
英文名称：DBCO-PEG-COOH，DBCO-PEG-ACID
CAS：-
分子量：400、600、1K、2K、3.4K、4K、5K、10K
纯度：95%以上
应用简介：
Ponsure’DBCO
(dibenzocyclooctyne) PEG acid, DBCO-PEG-COOH, is a heterobifunctional reactive
PEG derivative that can go Click Chemistry reaction without a need of any metal
catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and
azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction
that enables the conjugation of two molecules in aqueous solution. DBCO PEG
derivatives possess fast kinetics and stability in aqueous buffer. DBCO
reagents can be used to label azide-modified biomolecules spontaneously without
the need for toxic Cu catalysts. Carboxylic acid group, on the other hand, can
be activated to react with amines, hydroxy and other functional groups.
Copper-free
Click reaction procedures:
Prepare
the azide-containing reagents in reaction buffer.
Add
DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting
reagent to 1.5~3.0 mol equivalents of abundance reagent.
Incubate
the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
Purify
conjugates either by dialysis or size-exclusion chromatograph.
物理性质：
状态：淡黄色到白色固体，或者半固体，取决于分子量。
溶解性：溶于水，氯仿，DMSO等溶剂。
保持条件：-20℃长期保存，避光，干燥。
注意事项：取用一定要干燥，避免频繁的溶解和冻干。
反应性：DBCO于含有N3基团的反应。NHS可以和NH2。
技术指标：
纯度（PEG）：95%以上
取代率（DBCO）：90%以上
取代率（COOH）：95%以上
分散系数（PDI）：小于等于1.05
分析：COA、GPC、NMR、MALDI-TOF